Laser wavelength selection, a critical determinant of accuracy and data quality, is guided by factors such as resonance, fluorescence, and sample absorption. Ensuring the correct laser wavelength ...
Cy3 emits fluorescence with a peak wavelength at 563 nanometers (black line), while Cy5 at 670 nanometers (red line). In the experiment, Cy3 is attached to CB[7] and Cy5 to AdA. During autophagy, the ...